A novel ratiometric fluorescent probe for selective detection of bisulfite in living cells
نویسندگان
چکیده
A new type of ratiometric fluorescent probe, 8-(diethylamino)-2-methyl-4-oxo-4H-pyrano[2,3-b] chromen-10-ylium tetrafluoroborate (APCT), is reported. A new type of ratiometric fluorescent probe, 8(diethylamino)-2-methyl-4-oxo-4H-pyrano[2,3-b]chromen-10-ylium tetrafluoroborate (APCT), is reported. This probe can successfully detect bisulfite anions with a detection limit of 6.1 10 7 M based on Michael-type addition reaction in pH 1⁄4 7.4 Tris buffer solution containing 70% DMSO (v/v). The response time of the probe is approximately 5 min. Upon treating with bisulfite anions, the probe exhibits an obvious blue shift from 560 nm to 510 nm and the fluorescence intensity ratio at 510 nm and 560 nm (I510/I560) displays a good linear relationship with the concentration of HSO3 in the range of 20–120 mM. Additionally, the preliminary cell imaging experiments using HeLa cells demonstrate that the probe can be used to detect intracellular bisulfite anions.
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